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MedChemExpress
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Biosynth Carbosynth
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TargetMol
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MedChemExpress
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Janssen
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Bachem
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GenScript corporation
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Peninsula Laboratories
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Genemed Synthesis
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Henkel Corporation
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Qiagen
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Image Search Results
Journal: Pathogens (Basel, Switzerland)
Article Title: Mycobacterium tuberculosis Central Metabolism Is Key Regulator of Macrophage Pyroptosis and Host Immunity.
doi: 10.3390/pathogens12091109
Figure Lengend Snippet: Figure 3. fdr mutants are more immunogenic in MΦ and in vivo. MSD MULTIARRAY analyses of mycobacteria-infected THP-1 supernatants for (A) IL-1β, (B) TNF-α, (C) IL-8, (D) IL-4, (E) IFN-γ, and (F) IL-12. Supernatants were collected on day 4 for analyses. Data are from three independent infections; means and standard deviations are shown. (A–E) For fdr infections, p < 0.05, relative to BCG parent control. (G) fdr mutants enhanced M. tuberculosis-specific CD4 T-cell responses in vivo. C57BL/6 mice were subcutaneously immunized with fdr mutants or controls, and at 3 weeks post immunization, spleens were harvested and assayed for M. tuberculosis-specific CD4 responses (peptide-25) by IFN-γ ELISPOT. (H) fdr mutants enhanced M. tuberculosis-specific CD8 T-cell responses in vivo. C57BL/6 mice were subcutaneously immunized with fdr mutants or controls, and at 3 weeks post immunization, spleens were harvested and assayed for M. tuberculosis-specific CD8 responses (TB10.3/.4) by IFN-γ ELISPOT; 3 mice per group. (G,H) For fdr infections, p < 0.05, relative to BCG parent control.
Article Snippet: Then, 5 μg·mL−1 of peptide specific to mycobacterial CD4 (Peptide-25 (H2NFQDAYNAAGGHNAVF-OH, >85% purity, New England Peptide)) or
Techniques: In Vivo, Infection, Control, Enzyme-linked Immunospot